FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19708222
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC246030.12483622317629667No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT241030.12229921095875619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA67850.025.78359852
CCCTAAG73550.023.9453753
CCTAAGC73850.023.84809554
CTAAGCT81650.021.67696655
GCCCTAT53250.021.55062553
CCCGTAT17550.020.070081
TAAGCTC89350.019.90843856
CCTATGC57550.019.4778855
ACTATCG24500.019.38616458
CTACACG45000.018.68381357
CTATGCT60650.018.48277756
GTTCCTA36100.018.30132515
CCCTATG63550.018.28986554
AACGCTA46800.018.15053623
CTATCGG26250.018.09269559
AGTTACC36700.018.08620534
AGCCCTA113500.017.88280751
ACGCTAC47800.017.83292824
GTTTACG13800.017.0982866
GTCGGTT24450.017.0694481
Produced by FastQC (version 0.11.5)