FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17371212
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT332940.19166192894312728No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC281080.16180793832923115No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTCTCCAGCAACTGGAAG190920.10990597547252316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA77750.027.7745952
GTTCCTA47050.025.88957215
CCTAAGC84150.025.80191454
AGTTACC46400.025.62287134
CCCTAAG86300.025.0907453
CTAAGCT88900.024.28958355
CCCGTAT18100.023.3826961
TAAGCTC94150.023.06093856
CCTACTC55700.021.8690218
AGCCCTA109550.021.51654851
TATGCCG13500.020.98079546
TTCAACG107050.020.47088413
GTCGGTT21500.020.3732511
CAACGTT146200.020.31498715
TCAACGT109000.020.15878114
CGTTGTG147200.020.01668518
AACGTTG148400.019.89457116
ACCGGTG32950.019.6058528
ACGTTGT151300.019.55223817
CTACTCT63150.019.4291819
Produced by FastQC (version 0.11.5)