FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19600835
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCC439500.2242251414289238No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC352150.17966071343389198No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCAC260890.13310147246278028No Hit
GTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCAT220140.11231154182972307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA91450.026.8373452
CCCTAAG94250.026.03991553
CCTAAGC95550.025.6544354
GCCCTAT117000.025.08619753
CTAAGCT100750.024.41810655
TATGCTC122900.023.92971657
CCTATGC122300.023.82985755
CTATGCT122750.023.69449856
TAAGCTC109100.022.6304356
CCCTATG132150.022.2322954
CCGGCTT112000.019.9095542
ATGCTCC149300.019.89586658
GTTCCTA31700.019.6327415
GCCGGCT109150.019.2024331
TTCACGC100550.019.0379567
TTCAACG137100.018.26534813
GCTCCCC175850.018.1334759
AAGGGAC303700.017.68748527
CGGCTTC123850.017.575923
CACGCTC108850.017.5591839
Produced by FastQC (version 0.11.5)