FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15081011
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT177750.11786345093177109No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC161010.10676339935034859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA39500.025.61335215
AGTTACC40100.025.16022134
GCCCTAA53300.022.91244152
GCCCTAT43050.022.33776953
CCTACTC46400.022.0587518
CCCTAAG57350.021.29424553
CCTATGC45000.021.17307355
CCTAAGC58050.021.08821154
TTCGACG14150.020.6370756
CTACTCT51850.020.4227919
CTATGCT46400.020.21636256
CTACACG47550.019.29330457
TATGCTC49450.019.26783857
GCACTAC46650.019.09604525
CCCTATG51750.018.75337654
TACTCTC56000.018.6986920
AACGCTA50600.018.65417523
CGAGGCG46400.018.3727354
CTAAGCT67450.018.32423855
TCGACGA15550.018.2100127
Produced by FastQC (version 0.11.5)