FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13651183
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6900.028.21993448
AACGCTA28550.024.27783423
ACGCTAC28900.024.08596224
CTACACG28900.023.88630557
CGCTACC29600.023.21742425
GCCCTAA33950.021.2026752
CCCTAAG34700.020.40395253
CCTAAGC36250.019.61288854
CCCGTAT8800.019.1699351
GAAAACG36400.019.12312320
TACACGT37100.018.6862358
AAAACGC37400.018.6118121
ACGGATA32400.018.3937740
GCCCTAT28950.018.34241553
CCTACTA23100.018.13616846
GTCGGTT11750.018.1352331
ATTTCGC19000.017.69699318
GTCCGTT12250.017.6366181
TCACGCT42150.017.5640378
ACTATCG20750.017.34480758
Produced by FastQC (version 0.11.5)