FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14021164
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGTCTTGTTGGGGGTGAAGTAAAAGAAGGTTGCTTGGATCTCCTGAACCGACTTATTGTAC145680.10390007562852842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA22200.022.19230552
GTCCGTT13350.020.838421
AACGCTA20150.020.78544223
ACGCTAC20750.020.04248624
CCCTAAG25750.019.70498853
CCTAAGC26200.019.47914154
CGCTACC22300.018.91399825
CCCGTAT9950.018.7385351
ATTTCGC20500.018.56016518
ACGGATA33100.018.45047640
CTACACG22600.018.14392957
CGTATAA8500.017.3499033
ACTATCG15550.017.26384558
CTCGTAT20950.016.9517841
GTCGGTT12150.016.563451
CGCCCTA22900.016.49013549
CCTACTC23450.016.22530418
CGGATAG38100.016.18385141
CTAAGCT32000.016.1322355
ACGTTCG5050.015.7695156
Produced by FastQC (version 0.11.5)