FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23850458
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT264520.11090772344916815No Hit
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCACATTTTTTTCCAAAT262390.11001465883799799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA72000.023.4755752
CTACACG99100.022.47313557
CCCTAAG80650.020.77476953
CCTAAGC81650.020.44781954
CGAGCTA15200.019.98758711
AACGCTA115900.019.59658423
GCCCTAT53550.019.50006353
ACGCTAC117350.019.4303424
GTCCGTT24150.018.727381
TACACGT120100.018.6909458
CGCTACC122400.018.6527825
GCTCTAC121850.018.64025954
ACACGTT121950.018.28660859
CTAAGCT92000.018.2756955
TTACGAG18200.018.1515358
AAAACGC128050.017.9673321
GTTTACG20750.017.484526
TACGAGC18800.017.1015459
GAAAACG133900.017.09416420
CAACGTT158300.017.0306815
Produced by FastQC (version 0.11.5)