FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18861732
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATAT282380.14971053559662495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA46900.023.08293352
GTCCGTT17150.021.0301381
GCCCCGT32250.020.7169191
CCCTAAG53600.020.19751553
AACGCTA75450.019.46905523
CCTAAGC56150.019.43766254
CGAACGA10850.019.30532532
CTACACG75850.019.13401857
AACGAAT43800.019.13049139
ACGCTAC78000.018.90880224
CTAAGCT59900.018.81162555
CAACGTT77200.018.64494915
CCCGTAT18450.018.5869461
ACGAACG11750.018.57896831
GCGGACT50950.018.5252652
CGTTGTG76750.018.52368218
CGCTACC79700.018.39444425
ACGAATT45400.018.39115540
GCCGGCT88600.017.9178961
AGCCCTA66400.017.85989251
Produced by FastQC (version 0.11.5)