FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18183646
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGTCTTGTTGGGGGTGAAGTAAAAGAAGGTTGCTTGGATCTCCTGAACCGACTTATTGTAC230820.12693823889884348No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT189950.1044619984352973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA34500.021.5447315
GCCCTAA39100.021.35035552
CCTACTC36050.020.53657718
CTACACG70150.020.10003757
AACGCTA71350.020.00888623
ACGCTAC71900.019.7744324
GTCCGTT17000.019.4743211
CCCTAAG44150.019.24225653
GTCGGTT16900.019.239741
CGCTACC74150.019.09483725
CCTAAGC44500.018.95812854
TCACGCT77700.018.942588
AGTTACC39250.018.94089534
CCGGCTT84700.018.6655582
TTCACGC81500.018.0231767
TATGCCG8250.017.88083848
GCCGGCT82700.017.656911
CTAAGCT48950.017.17440255
GAAAACG83750.017.15156420
AAAACGC83950.017.07580221
Produced by FastQC (version 0.11.5)