FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21992412
Sequences flagged as poor quality0
Sequence length65
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5269432.396021864268458No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT624050.28375696126463984No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTG277090.12599345628846895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT33950.029.3632016
GACCGCG34850.028.8587885
TTCGACG26800.027.1824536
GTTCGAC28550.026.1361055
GCCGGCT237850.026.0163881
AGACCGC43600.024.352444
CCGGCTT285800.023.6835582
AACGACG25500.023.36391316
TCGACGA33000.022.8798147
TTCACGC309850.022.359297
CTACCGA6100.022.253139
CGGCTTC323350.022.0369033
TCACGCT312750.022.0010748
CCGCGTT45450.021.9335677
CAGACCG49450.021.8890173
CTACACG284950.021.84384757
TAACGCG1255.7330726E-721.24044852
TACACGT292100.021.22802558
ACGCTAC326800.021.19181624
CGACGGT27050.021.04457918
Produced by FastQC (version 0.11.5)