FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39394642
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG50750.028.1858625
ACCGCGT51850.027.4741336
AGACCGC65050.022.3071164
CAGACCG68350.022.0068173
CCGCGTT65500.021.8386087
CGCGTTC72050.019.935158
CCGTACG68000.018.9538824
TTCGACG36900.018.8630226
CGTACGC68400.018.8430395
CTACACG189250.017.56734857
AACGCTA212500.017.08679423
ACGCTAC212500.016.93387224
CGCTACC219250.016.45318425
CGGTATA48850.016.24271220
TCCGTAC81000.016.0575473
GTACGCC82100.015.84240156
GTTCGAC46000.015.8367025
CGTTACC33550.015.74371545
TACACGT213950.015.55265758
TCGACGA45450.015.5091697
Produced by FastQC (version 0.11.5)