FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19914582
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4356942.187813934532997No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT625680.31418183921711235No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC260590.13085386376676145No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC233550.1172758735282518No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATAAACAATGCACTAAA224830.11289717253417622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC34100.030.2719065
TTCGACG35100.029.4094586
CCGATTA32550.028.36634858
TCGTTAC35000.028.15942444
CGATTAT33850.027.53852759
CGGTATA46550.027.37454420
TCGACGA38950.026.1996047
ACCGATT35350.026.12029657
AGTTCGA41650.025.5633814
AACCGAT36700.025.56054156
GCCGGCT274100.024.4729061
TCGACAC39400.024.26004635
AGACCGC79350.024.1225874
TCGCCTA43200.024.12
GACCGCG80200.023.6094995
CGACGAG43950.023.4874178
GCACTAA78450.023.46398258
ACCGCGT82000.023.1272096
CCGGCTT313700.022.987432
ATAGAGT58850.022.80495524
Produced by FastQC (version 0.11.5)