FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15444311
Sequences flagged as poor quality0
Sequence length65
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7471304.8375741721336745No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTG398740.2581792091599295No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGAC281880.1825138071876434No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT230850.14947251450712176No Hit
CTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAATATGCTTAAATTCA199370.12908960457996474No Hit
CGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTC163720.10600667132382921No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGC162940.10550163098891237No Hit
GTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTCTCCTCCGCTGACTAATATGCTTAA156370.1012476373986512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG355.740758E-542.1339048
TCGCGTG1850.033.4795882
TATGCCG11350.025.4767646
GCCGGCT82950.025.4366661
CCGGCTT97500.023.9581412
CGCGTGA2300.021.7998543
TTCACGC109850.021.425587
TCACGCT107650.021.2880928
CCCGTTT29150.021.0864331
CCCGTAT11750.020.623121
CGGCTTC117550.020.5240573
GTCGCTA3900.020.4586751
GCCCTAA36200.019.88419352
CGACAAT20050.019.8624854
GCCCTAT32700.019.84692453
GCACTAA55200.019.71923858
GTCGCGA2103.8198777E-1119.7009491
CACGCTC117150.019.6876629
ACGAATT45950.019.52245340
CCCTAAG39100.019.46530753
Produced by FastQC (version 0.11.5)