FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18049009
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA296100.16405332835725217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG20250.028.1098825
ACCGCGT22400.025.5435036
AGACCGC27800.021.7488254
CCGCGTT26800.021.3497927
CAGACCG30600.020.3370423
CTACACG80650.018.72839757
TCGTTAC12400.018.56122644
CCGATTA12350.018.39224458
AACGCTA93500.018.26567623
ACGCTAC95200.017.93930824
CGCGTTC32150.017.797038
GCCCTAA52600.017.61113552
CGCTACC97600.017.37728925
CGTACGC21350.017.2678745
AAAACGC102600.016.58835421
ACACGTT91150.016.57011259
CCGTACG22500.016.5163764
TACACGT92050.016.44010458
CGTTACC13550.016.33207145
CCCTAAG58250.015.80128853
Produced by FastQC (version 0.11.5)