FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19773082
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT447250.2261913443741345No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC212330.10738336087414192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT51750.032.770826
GACCGCG52050.032.6386035
AGACCGC55450.031.1692184
CCGCGTT60900.027.8470557
CGCGTTC61650.027.6518048
CAGACCG63400.027.5398943
TCAGACC90150.021.3310242
GCCCTAA59650.020.22777752
GCGTTCT86250.020.0043989
TTCGACG15150.019.6624936
CTACACG194000.019.09955257
CTCAGAC114200.018.860731
TTCACGC215500.018.5994857
TCACGCT216500.018.4727068
ACGCTCA226200.017.98044410
AACGCTA216950.017.96039423
ACGCTAC217300.017.93110324
TACACGT208550.017.86563958
CCGGCTT218950.017.8619292
ACACGTT209050.017.7946459
Produced by FastQC (version 0.11.5)