FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32037038
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT890330.2779064656351814No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTC326090.10178531485963216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG82200.031.2160365
ACCGCGT85150.030.7234026
AGACCGC91200.028.6852824
TTCGACG37550.027.6479576
CAGACCG98250.026.9871793
CCGCGTT107100.024.3991457
GTTCGAC42650.023.8578015
TCGACGA45950.022.6578947
CGGTATA58200.022.04696520
CGCGTTC119450.021.9011968
CGACGAG47550.020.8410268
TCAGACC149000.020.2695472
GCCGGCT414400.019.950141
CCGATTA24700.019.22852758
CCGGCTT486050.019.1265032
AGTTCGA57050.018.869814
TCACGCT512350.018.8470028
TTCACGC512300.018.8142997
CTACACG470300.018.81164457
GCCCTAT81550.018.66595853
Produced by FastQC (version 0.11.5)