FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14975998
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG42100.08.373013594
GATCGGA140050.08.15503494
GTCGTGT51500.07.5748494
ATCGGAA131800.07.48870194
TCGTGTA43700.07.313580594
AGATCGG159250.07.17182194
CGTCGTG59850.06.98921694
AGCGTCG68750.06.83643894
GCGTCGT58100.06.71435994
TCGGAAG152750.06.61545794
AGAGCGT91650.06.461609494
CGTGTAG50950.06.180636494
GAGCGTC100700.06.020920394
AAGAGCG114950.05.96961794
CGGAAGA157350.05.91426994
AAGATCG56800.05.792317494
Produced by FastQC (version 0.11.5)