FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17806930
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT52200.08.01356794
GATCGGA116600.07.296025894
AGATCGG136150.07.284020494
ATCGGAA112450.07.27270794
AGCGTCG62550.07.13842894
TCGTGTA40550.07.070421794
GTCGTGT50000.06.674130494
TAGATCG39550.06.238910792-93
CGTGTAG48400.06.214997394
CGTCGTG54150.05.945495692-93
AAGAGCG108100.05.869679594
CGGAAGA147350.05.486364494
AGAGCGT89600.05.35055194
AAGATCG56750.05.300544394
TCGGAAG145350.05.060521692-93
Produced by FastQC (version 0.11.5)