FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21383647
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA157650.08.46703294
AGATCGG180600.07.859520494
ATCGGAA147750.07.698298594
AGCGTCG74950.07.65059394
AAGAGCG134950.07.03534494
GCGTCGT66400.06.441398694
TAGATCG53750.06.295944794
TCGGAAG183550.06.094378594
GAGCGTC120000.05.95345794
AGAGCGT107400.05.798320392-93
TCGTGTA53550.05.705066794
GTCGTGT65050.05.491274494
CGTCGTG69600.05.46994194
CGGAAGA184000.05.38978694
AAGATCG72800.05.229504694
Produced by FastQC (version 0.11.5)