FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28614456
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT92100.08.67554994
GATCGGA220200.08.1536894
TCGTGTA68250.07.7818694
AGATCGG252800.07.47406194
ATCGGAA205800.07.39958494
CGTCGTG93700.07.27337694
AGCGTCG109850.07.10257294
TAGATCG72800.06.972684494
GTCGTGT85000.06.690738794
TCGGAAG251650.06.518326894
AAGAGCG190100.06.082198694
AGAGCGT156100.05.66060194
CGGAAGA257750.05.634656494
GAGCGTC171200.05.40841694
CGTGTAG82100.05.266866794
Produced by FastQC (version 0.11.5)