FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24502846
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA199300.08.34827394
ATCGGAA187500.08.12165394
TCGTGTA62250.07.92776294
GCGTCGT89200.07.903638494
AGATCGG233900.07.69607394
CGTCGTG89500.07.036916794
GTCGTGT79050.06.95640194
TAGATCG64550.06.8805692-93
AGCGTCG103300.06.369655692-93
AAGAGCG176600.06.17444594
TCGGAAG228550.06.04598194
AGAGCGT140100.05.971487594
GAGCGTC156100.05.50996594
CGGAAGA237000.05.413958594
CGTGTAG75500.05.35367794
Produced by FastQC (version 0.11.5)