FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27291103
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA268200.08.8670594
GATCGGA287500.08.09197994
AGATCGG333400.08.02110394
TAGATCG89400.07.833144794
TCGGAAG313250.06.856658594
AAGATCG112950.06.449601794
ACACGTC126000.06.30381794
CGGAAGA311200.06.146703294
ACGTCTG121650.05.833809494
CACACGT169700.05.372882494
Produced by FastQC (version 0.11.5)