FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13492968
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC221350.09.19394994
CGTCTGA165950.09.00624294
ACGTCTG191050.08.56102894
CACGTCT215850.08.01289694
CACACGT261200.07.683311594
GTCTGAA190450.07.280056594
GATCGGA589100.07.17239894
AGAGCAC424100.07.1480194
ATCGGAA545600.07.14124494
GCACACG320500.07.009605494
TAGATCG173300.06.91568794
CGGAAGA512850.06.836631394
TCTGAAC173100.06.815070694
AGATCGG642800.06.792560694
TCGGAAG535200.06.779457694
CACACTA41950.06.72222494
TGAACTC165900.06.714221594
CAGATCG209650.06.456413394
CTGAACT184350.06.450168694
GAGCACA408350.06.39935194
Produced by FastQC (version 0.11.5)