FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21379222
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA171700.08.23928994
ATCGGAA511150.07.48461794
TAGATCG163550.07.471647794
ACACGTC220950.07.274877594
CACACGT267550.07.114487694
ACGTCTG197100.07.05827694
CACGTCT223450.07.046249494
GATCGGA552750.06.94683394
AGATCGG609850.06.78192894
TCGGAAG528500.06.598610494
TCTGAAC205300.06.432961594
CGGAAGA513900.06.292212594
AAGATCG160550.05.986451692-93
AGCACAC390450.05.946413594
CAGATCG221300.05.904144394
GTCTGAA215600.05.798644594
GAGCACA440800.05.70434594
GCACACG342150.05.700659894
AGAGCAC445800.05.68253794
AAGAGCA518300.05.640307494
Produced by FastQC (version 0.11.5)