FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21379222
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA173500.08.91360594
AGCGTCG277150.07.98845894
CGTCGTG232600.07.921971394
GTCGTGT200650.07.70750294
ATCGGAA512000.07.49164494
CGTGTAG185850.07.334869494
GCGTCGT245400.07.31721494
GTAGGGA179000.07.19539294
AAGAGCG402050.06.95654894
GATCGGA550000.06.922759594
GTGTAGG204650.06.91371894
TAGGGAA160150.06.809543694
GAGCGTC356150.06.7840294
AGATCGG610400.06.753703694
AGAGCGT348650.06.74120194
TCGGAAG534550.06.595228794
TAGATCG160300.06.539254794
AAGATCG160900.06.36879794
GAGTGTA77000.06.22682194
CGGAAGA521500.06.13832394
Produced by FastQC (version 0.11.5)