FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20439921
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA246800.08.26477894
ACACGTC324750.08.10448694
GTCTGAA285950.08.08651994
CACGTCT313950.07.949149694
ACGTCTG281950.07.72603892-93
CACACGT379300.07.69476694
ATCGGAA757250.07.11886594
TCTGAAC262600.07.01581394
AGATCGG889750.06.8193894
TCGGAAG749300.06.811782494
GCACACG470000.06.69982794
GAACTCC245100.06.673022794
TAGATCG243600.06.636938694
GATCGGA815800.06.60794494
CTGAACT279600.06.45476994
AGCACAC524600.06.44150494
GAGCACA584800.06.43741994
CGGAAGA714850.06.34452494
TGAACTC253350.06.158909394
AGAGCAC601450.05.91137792-93
Produced by FastQC (version 0.11.5)