FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4180420
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA13050.010.44565794
AGATCGG50850.08.59686894
TAGATCG15700.07.934231892-93
TATCTTA7603.0446277E-47.4212932
AGCGTCG26550.07.08180294
GTTATAT6650.0051262527.06874281
GCGTCGT21350.07.04531494
ATCGGAA40150.07.02447694
GATCGGA44600.06.955964694
GTCGTGT18250.06.439257692-93
AGGGTAT9505.4453034E-46.43255571
TCGGAAG51350.06.407754494
CGTGTAG16951.3080171E-76.10100394
GTTCTAC10803.8481885E-46.09351871
CGTCGTG21700.06.065365392-93
AGAGCGT33250.05.93753194
AAGAGCG39900.05.772599794
TTAGATC7807.5860826E-65.7251692-93
TACTGAT9950.00638581075.66832164
CAGATCG30700.05.359056592-93
Produced by FastQC (version 0.11.5)