FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6344339
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA35700.09.61233294
GTAGGGA94950.09.45611194
GTGTAGG118550.09.35803494
AGTGTAG34050.09.111732594
CGTCGTG159300.09.08885394
TAGGGAA86950.09.02861394
AGCGTCG193400.08.92038894
CGTGTAG118450.08.87034692-93
TGTAGGG116600.08.82916794
GTCGTGT140900.08.57425394
TCGTGTA125400.08.43449694
GCGTCGT172450.07.95964794
AAGAGCG257250.07.49208494
GTAGATC43050.07.4252394
AGAGCGT230700.07.29473694
GAGCGTC222100.07.217386794
AGAGTGT56600.07.05954494
AAAGAGT69500.06.696140394
CGGAAGA332300.06.620482494
GATCGGA386150.06.46416894
Produced by FastQC (version 0.11.5)