FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10175235
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG143100.08.37500294
CGTCTGA127500.08.36758394
ACACGTC165650.08.05770494
CACACGT192800.07.995602694
CACGTCT159500.07.86746794
GCACACG223300.07.61910994
AGCACAC267400.07.381969594
TCGGAAG399400.07.1545194
CGGAAGA377900.07.051646794
GTCTGAA155050.06.97172894
ATCGGAA406750.06.805689394
GATCGGA438300.06.78760694
AGATCGG474250.06.451460494
TCTGAAC139250.06.41272794
GAGCACA291750.06.40333792-93
TGAACTC137700.06.31425594
AAGAGCA360550.06.139595594
AGAGCAC306300.06.091490792-93
CAGATCG156250.06.075970694
TAGATCG127400.05.939317792-93
Produced by FastQC (version 0.11.5)