FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22178486
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA220450.09.16871994
GTCGTGT260750.08.83328294
TAGGGAA170950.08.49649394
CGTGTAG227550.08.40751994
GCGTCGT326600.07.858267394
GTAGATC92950.07.737352494
CGTCGTG307350.07.631635794
AGCGTCG365850.07.490586394
GTAGGGA202300.07.45863794
GATCGGA705950.07.430885394
GTGTAGG242900.07.334356394
TAGATCG186700.07.27618794
GAGTGTA75350.07.111675394
ATCGGAA650050.07.086464494
AGAGCGT445350.07.04003394
AGATCGG778900.06.96425194
AAGAGCG508850.06.81737194
TCGGAAG668300.06.65380494
CGGAAGA653700.06.622644494
GAGCGTC444950.06.602662694
Produced by FastQC (version 0.11.5)