FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6624995
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA123250.09.19041594
GTCTGAA121950.08.98005994
ACACGTC163900.08.86103894
CACGTCT154250.08.47080494
ACGTCTG141200.08.35495494
CACACGT194250.08.00888394
ACTAATA6000.00187792237.83334453
TCTGAAC110250.07.673596494
TGAACTC106500.07.54660494
GCACACG233700.07.260282594
CTGAACT114850.07.161633594
AGAGCAC286700.07.01650994
GAACTCC107600.06.901601394
AGCACAC246450.06.684175592-93
CGGAAGA364800.06.62236994
GTTAGAT11400.06.59635292-93
TGGCATA22303.6379788E-126.533740594
ATCGGAA388200.06.477438494
AGATCGG452000.06.395017694
AACTCCA94250.06.3831394
Produced by FastQC (version 0.11.5)