FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26101203
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC257500.08.32288494
ATCGGAA596100.07.5926594
CGTCTGA193950.07.46357994
GATCGGA650000.07.2884294
CACGTCT258200.07.098957594
AGATCGG720700.07.095133394
TCGGAAG616800.06.933990594
ACGTCTG227700.06.852692694
CGGAAGA597750.06.84833294
ACGAATC29400.06.714105694
TAGATCG174500.06.65254494
GTCACGA54700.06.615910594
CACACGT319550.06.53025894
CACGAAT38450.06.111669594
TCTGAAC219750.06.031237694
AGAGCAC529300.06.020242794
GTCTGAA233250.06.004554794
AGCACAC472450.05.988620894
GAGATCG311800.05.984123794
AAGATCG179700.05.96311294
Produced by FastQC (version 0.11.5)