FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26101203
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA192200.09.0249294
GTCGTGT232250.08.72352494
AGCGTCG330250.08.18456294
GCGTCGT294250.08.003715594
CGTGTAG210750.07.51679594
CGTCGTG274800.07.49251894
ATCGGAA598550.07.49235594
TAGGGAA167950.07.389147394
AGAGCGT411000.07.297082494
GATCGGA653600.07.228104694
AGATCGG722200.07.12082494
TAGATCG168450.07.032341594
TCGGAAG634950.06.870350494
GTAGGGA197750.06.65597994
AAGAGCG480000.06.59089394
GAGCGTC414700.06.529183494
CGGAAGA624450.06.45139494
CAGATCG288950.06.24710794
GTGTAGG236550.06.160403394
GAGTGTA80250.05.9748394
Produced by FastQC (version 0.11.5)