FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5643703
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG41850.036.202156145
CCGTATA58400.027.43235145
CGCCGTT22550.025.7174145
TCTCGGG87100.025.55076145
CGGTGGG116250.022.324085145
AGAGCGG81750.021.991129145
GCCGTAA36400.021.707447145
TCGGAAC10550.019.926414145
GGGAAAA128250.018.087402145
TAGATCA81350.017.198214145
TCGCCGG133400.015.595872145
CGCCGTG9950.014.571038145
TGTAGAA105200.013.5748205145
GTATCAA64550.013.251633145
GTAGATA204300.012.915661145
GTCGCCC54250.012.827887145
GCCGGAA19250.011.673862145
CGGAAGC72950.011.129518145
CCGGATA29300.010.638598145
TCGTGTT138800.010.288696145
Produced by FastQC (version 0.11.5)