FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18374991
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG91550.038.246258145
CCGTATA114700.028.25168145
TCTCGGG157650.026.624702145
AGAGCGG186800.026.428373145
CGCCGTT38650.024.195824145
GCCGTAA70550.021.886826145
CGGTGGG222200.021.695906145
TCGCCGG212050.018.32427145
CGGAAGC150150.017.332823145
TCGGAAC33800.017.158268145
CGTCGTT19500.015.614025145
TCGTGTT270300.014.777661145
GTAGATA455900.013.436536145
TAGATCA230750.012.786534145
TCGGAAA55550.012.658663145
CCGGATA53550.012.183812145
GGGAAAA476850.012.0556755145
GTCGCCC125550.011.894623145
TCGCGGG22900.010.130121145
GAGCGTG47650.09.888961145
Produced by FastQC (version 0.11.5)