FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10451396
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG39050.044.739784145
AGAGCGG80900.029.839643145
TCTCGGG69800.027.210955145
CGCCGTT19600.026.630194145
CCGTATA53650.025.80843145
CGGTGGG92900.023.64421145
TCGCCGG96850.018.637928145
CGGAAGC67500.017.720587145
TCGGAAC15400.016.946487145
GCCGTAA33600.015.965788145
TCGTGTT143900.015.66742145
TCGGAAA23700.014.070431145
GTAGATA239950.013.7463875145
CGCCGTG10152.1827873E-1112.855955145
AGCGCGG12300.012.376905145
GAGCGTG25000.012.178875145
GTCGCCC51200.011.751844145
TAGATCA125300.011.455447145
GCCGGAA17900.011.339735145
TCGCGGG11602.237357E-1011.248961145
Produced by FastQC (version 0.11.5)