FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6483321
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209460.32307516471882236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG28450.040.51298145
TCTCGGG30750.031.117746145
AGAGCGG63550.028.516996145
TCGCCGG36500.026.612843145
CCGTATA27150.022.427908145
TCGGAAC8900.019.54792145
GCCGTAA11150.018.853954145
GAGCGTG12550.016.173113145
CGTCGTT5904.9258233E-915.972418145
CGGTGGG68800.015.909915145
TCGTGTT89800.013.965261145
TAGATCA60000.013.048237145
CGGAAGC60850.012.985099145
GGGAAAA102300.012.896596145
CGAGCGG5350.001512709810.839656145
CGCCGTT9602.4529072E-710.571488145
GGTCGAC4850.00902046810.462504145
GTAGATA135200.010.4017145
GCGTATA6506.73137E-410.037105145
CGGAAGG14501.0004442E-109.998649145
Produced by FastQC (version 0.11.5)