FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8396788
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG83500.030.039297145
CCGTATA64700.023.641682145
TCTCGGG138550.022.969849145
AGAGCGG155550.019.201155145
CGCCGTT33850.018.20373145
CGGTGGG203300.017.615288145
GCCGTAA40350.017.247568145
TCGCCGG178050.016.082563145
TCGGAAC19600.015.534362145
GAGCGTG26450.014.800222145
TAGATCA115750.014.029016145
CGGAAGC130350.013.458743145
GTAGATA245150.013.070449145
CGTCGTT12700.012.557961145
TCGCGGG26000.011.98934145
GGGAAAA197800.011.581397145
TCGTGTT210950.011.443658145
GAGTGTT188100.010.598491145
CGGTGCG5550.002052826610.44954145
GTCGCCC75400.010.19142145
Produced by FastQC (version 0.11.5)