FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20667902
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATGGCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1461880.7073190108991226No Hit
CATAATGGCGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1010140.48874820482504705No Hit
CATAATGGCGAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC311150.15054745276032372No Hit
CATAATGGCGAGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC258670.12515542216137854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGA365300.0104.576525
TGGCGAG264850.098.31526
GCGAGTA78600.094.356758
AATGGCG408650.093.7740254
CGAGTAC80000.092.935989
GCGAGGC201650.092.463098
TGGCGAT132950.091.5297246
CGAGGCG209300.089.346439
CGATGAA137000.088.654159
GGCGATG139050.087.473337
GGCGAGG217100.085.860327
TAATGGC453350.084.774573
GGCGAGT87800.084.4744347
GCGATGA147100.082.3583158
CATAATG500550.076.993491
ATAATGG511250.075.420462
ATAACCG172400.054.367740-41
TACGCTC168550.053.928852118-119
CGGCATA171100.053.829823114-115
AGTCGCC172850.053.8262328-29
Produced by FastQC (version 0.11.5)