FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22248948
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGGCCTTCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT559660.2515444775186674No Hit
AGCTGGCCTTCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA432690.19447661075930422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG76250.099.310459
GCCTTCG133000.074.150626
CCTTCGG105200.072.205097
CTTCGGC122150.062.2362948
TCGTACG21500.052.84973510-11
TTCGTAC46100.049.9363069
TACGCTC73250.049.770958118-119
GGCCTTC315200.049.7626575
TTCGCGT58350.049.5971418-19
CTTCGTA47300.049.1724668
TCGGCGT78100.048.33130610-11
GTTCGCG59350.048.26032618-19
TCGCGTT59800.048.04789420-21
CGCGTTT60450.047.87567520-21
ATAACCG79800.047.4823440-41
CGGCATA79650.046.86027114-115
GTACGCG62600.046.17720836-37
ATACGCT79050.046.081547118-119
AGCTGGC339000.046.0039441
GCATACG82050.045.814518116-117
Produced by FastQC (version 0.11.5)