FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23190908
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGCCCGCCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1176940.5075006118777238No Hit
TAGAGCCCGCCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA933890.4026966085157166No Hit
TAGAGCCCGCCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC435690.18787103980577216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGC349150.0101.221234
GCCGTAC78750.099.6364449
CCCGCCG236600.096.4307566
CGCCGTA83700.094.383618
CGCCGGC160300.093.183118
GCCGGCG161200.092.441449
GAGCCCG384350.091.808783
CCGCCGT86850.090.5396357
CCGCCGG168150.089.177047
TAGAGCC398950.088.963321
GCCCGCC505350.070.0990145
AGAGCCC527750.067.279652
CCGCCTG229400.054.7099237
CCGTACG71850.054.60243610-11
GCGATAA141600.053.2126336-37
CGATAAC141300.053.0955838-39
CGTACGC74950.052.5027710-11
TTCGCGT120300.052.1446918-19
ATAACCG144600.051.905140-41
TACGCTC141250.051.469627118-119
Produced by FastQC (version 0.11.5)