FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22691862
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCTTCGATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1104100.48656209878237405No Hit
GGTCCTTCGATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA764500.3369049221258264No Hit
GGTCCTTCGATGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC370580.16330964819017496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT282550.0108.285515
CCTTCGA286250.0106.7455144
TCCTTCG290900.0104.890913
CGATTGA97150.099.737458
TCGATGG148200.099.371827
TTCGATG212850.097.857616
TCGATTG102150.095.084247
CGATGGC159600.091.793418
GGTCCTT364750.083.817241
GATGGCG178450.082.030449
TCGATGT72600.081.1071857
GTCCTTC397800.076.898232
TTCGATT129550.075.33966
CGATGTA94550.062.595278
GCGATAA142450.053.79934736-37
TACGCTC137450.053.136005118-119
TTCGCGT94650.052.3833518-19
CGATAAC143150.052.2083138-39
ATAACCG143200.052.1295640-41
CGGCATA140950.051.67613114-115
Produced by FastQC (version 0.11.5)