FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23412104
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTAAATTACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1977890.8448151434830462No Hit
CCGTAAATTACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1440470.6152672139163571No Hit
CCGTAAATTACGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC560340.2393377374370112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG217650.0114.814749
TTACGGC225150.0111.016558
CCGTAAA461850.0110.493971
CGTAAAT468950.0108.8337552
ATTACGG235550.0106.287187
TACGTAC85550.094.2851649
TTACGTA93950.085.601948
GTAAATT669700.076.422853
AATTACG479250.069.192416
TAAATTA872500.058.815414
AAATTAC875150.058.656085
ACGGCGT218850.057.03008310-11
ATAACCG223150.056.0966140-41
ACGTACG72150.055.6560610-11
CGATAAC224950.055.64547738-39
TTCGCGT161300.055.55778518-19
GCGATAA226500.055.47226336-37
TACGCTC219850.055.216442118-119
TCGCGTT161950.055.02512420-21
CGCGTTT163600.054.72469720-21
Produced by FastQC (version 0.11.5)