FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19873523
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTCGATATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1419120.7140757076639104No Hit
GTAGTCGATATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA934720.4703343237130125No Hit
GTAGTCGATATGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC463670.23331042010015035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTCG313700.0115.813791
TAGTCGA315700.0115.130072
GTCGATA317250.0114.8944
AGTCGAT324900.0112.2623
CGATATG227000.0111.128866
TCGATAT329550.0110.677965
TATGGCG173350.0107.047699
CGATATT116850.096.333936
ATATGGC249000.074.764098
GATATGG250000.074.5126347
CGATAAC168400.055.03354338-39
GCGATAA170350.054.8227336-37
ATAACCG171500.054.1076840-41
CGGCATA171150.054.0045114-115
TAAGACG175450.053.4635864-65
AGCGATA173450.053.44839536-37
TACGCTC172650.053.436672118-119
TTCGCGT106450.053.4041718-19
GATATTG212850.053.3324557
CGACCAA174350.053.13929770-71
Produced by FastQC (version 0.11.5)