FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24635192
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGATTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1747500.7093510779213736No Hit
GTCTTTGATTATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1062360.4312367445725611No Hit
GTCTTTGATTAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC566940.2301341917692381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG201900.0106.7277459
GATTAGG284350.076.1788567
ATTAGGC287400.075.3287058
TGATTAG398550.072.771076
TTGATTA590850.071.883295
GTCTTTG662850.064.006191
CTTTGAT724350.058.8341563
TCTTTGA744750.057.1928372
AGTCGCC197150.054.89155628-29
TACGCTC197450.054.852592118-119
GCGATAA199250.054.479936-37
CGATAAC196850.054.45051638-39
GCGTCCA202300.054.12689212-13
CGGCATA199000.053.857906114-115
ACCGGAG203100.053.7848242-43
TTCGCGT127500.053.7773618-19
CGGAGTA202300.053.758344-45
ATAACCG201050.053.4902140-41
GAGTCGC199300.053.4784928-29
CGACCAA201750.053.31746770-71
Produced by FastQC (version 0.11.5)