FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20725116
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACGCAAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1604350.7741090568564248No Hit
CTCCACGCAAGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1055930.5094929263604604No Hit
CTCCACGCAAGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC377410.18210272019707877No Hit
CTCCACGCAAGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC210630.10163031174349037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACGC377300.0104.78652
CCACGCA391350.0101.620563
ACGCAAG391300.0101.557515
CTCCACG393850.0100.524251
CACGCAA419150.094.852214
CGCAAGG288750.092.824446
AAGGGCG212400.092.0174949
CGCAAGT182350.071.454226
GCAAGGG322400.060.861857
CAAGGGC334250.058.7041478
GCGATAA176950.055.28280336-37
ATAACCG178200.054.71122740-41
CGGCATA179550.053.880745114-115
TTCGCGT121100.053.69930618-19
AGCGATA181950.053.53470636-37
CGGAGTA183000.053.01069344-45
CGATAAC183750.053.01028438-39
GAGTCGC183750.052.76143328-29
GTTCGCG123000.052.7488818-19
TAAGACG186300.052.72867264-65
Produced by FastQC (version 0.11.5)