FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20725116
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACGCAAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1428730.6893712923006077No Hit
CTCCACGCAAGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA989420.4774014292609991No Hit
CTCCACGCAAGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC358550.17300265050386207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACGC365300.0105.468772
CCACGCA382150.0100.678243
ACGCAAG383650.0100.08715
CTCCACG387550.099.567131
CACGCAA412850.092.95324
CGCAAGG281950.091.681396
AAGGGCG212450.089.709459
CGCAAGT176600.071.5364466
GCAAGGG315750.060.7332847
CAAGGGC331550.057.8606228
GCGATAA174600.054.9983936-37
ATAACCG174700.054.56331340-41
TTCGCGT118250.053.95303718-19
CGGCATA172850.053.949078114-115
AGCGATA179000.053.39726336-37
TCGCGTT118850.053.38209520-21
GTTCGCG120650.052.8797918-19
CGATAAC181350.052.6588738-39
GTACGCG120050.052.6160836-37
TAAGACG181950.052.31320664-65
Produced by FastQC (version 0.11.5)