FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19968901
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCGCTGGTTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1403390.7027877998894381No Hit
CAGCGCTGGTTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA890990.446188801276545No Hit
CAGCGCTGGTTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC441930.22130912462333305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCT327750.0105.917941
AGCGCTG328200.0105.6436162
GTTGGCG165600.0105.154929
GCGCTGG341700.0101.414533
CGCTGGT382550.090.8152544
GGTTGGC215650.080.887568
CTGGTTG301550.078.245736
GCTGGTT486500.071.484235
TGGTTGG273000.064.30937
GTTGTAC102000.059.7264989
ATAACCG153950.056.4633440-41
CGATAAC154750.056.0180138-39
AGTCGCC157200.054.97147428-29
GCGATAA159400.054.51381336-37
GAGTCGC159950.053.97056228-29
TAAGACG161850.053.96904464-65
TTCGCGT103950.053.82737418-19
GTTCGCG104500.053.4871418-19
CGGCATA159950.053.319572114-115
TTGGCGT163500.053.2531710-11
Produced by FastQC (version 0.11.5)