FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19968901
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCGCTGGTTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1269420.635698479350466No Hit
CAGCGCTGGTTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA889790.4455878668535639No Hit
CAGCGCTGGTTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC435400.2180390398049447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCT324000.0106.263831
AGCGCTG327700.0105.221912
GTTGGCG158750.0104.846629
GCGCTGG341750.0100.8786243
CGCTGGT378900.091.2117164
GGTTGGC211900.078.744898
CTGGTTG295950.078.417316
GCTGGTT481850.071.87275
TGGTTGG265400.063.1785857
GTTGTAC108400.059.3221479
ATAACCG148700.055.8947940-41
CGATAAC149350.055.8498638-39
AGTCGCC148450.055.00619528-29
GCGATAA151950.054.97101236-37
TTCGCGT104350.054.5849318-19
GAGTCGC150450.054.1959128-29
TTGGCGT154350.053.9562610-11
CGGCATA150150.053.876076114-115
GTTCGCG106150.053.8274518-19
CATACGC151000.053.55046116-117
Produced by FastQC (version 0.11.5)