FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004838673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004838673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8505336
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG226680.2665150442028392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG37000.028.409058145
TCTCGGG51400.020.73217145
CCGTATA32750.020.585539145
AGAGCGG67500.019.760803145
CGCCGTT15300.018.00457145
CGGTGGG81250.016.149006145
GCCGTAA22600.013.79274145
TCGCCGG57950.012.759607145
TCGTGTT106450.012.189838145
GTAGATA153250.010.028269145
CGGAAGC55100.09.604214145
TAGATCA83950.07.771634145
GGGAAAA193150.07.6188936145
TCGGAAC14558.263985E-67.47341145
AGCGCGG14851.1020822E-57.3224325145
GAGCGTG23954.1836756E-107.2643414145
GCCGGAA16506.3844564E-67.0295353145
GTAGGGG70800.06.962517145
CGTCGTT12556.660264E-46.931514145
GGTCGCC321650.06.4457436145
Produced by FastQC (version 0.11.5)